The Vcheck H utilizes three different testing methods to detect 35 parameters between white blood cells, reticulocytes, red blood cells, platelets and hemoglobin.

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6-Diff of WBC and Reticulocyte Analysis: Fluorescence

• Fluorescence-stained cells pass through the flow cytometer.

• Each cell moves through a multi-angle laser, and the system analyzes its size, complexity/granularity and fluorescence intensity.

• Allows the specific identification and classification of each cell.

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RBC and PLT Measurement: Sheath Flow Impedance

• A small electric current flows through a tiny aperture between two electrodes.

• When a cell passes through the aperture, it temporarily blocks the current, creating resistance known as impedance.

• By measuring this resistance, we can determine the size of each cell. Each cell passage generates a pulse, and by collecting these pulses, we obtain a graph displaying cell size and count.

• Compared to traditional impedance methods, by using the sheath flow, this approach is more accurate while requiring less maintenance and consuming less diluent.

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Hemoglobin Measurement: Colorimetry

• Cells are broken down through lysis, releasing hemoglobin.

• The released hemoglobin reacts with a specialized reagent, causing a color change. The degree of color change is proportional to the amount of hemoglobin and is measured by the light absorbed at a specific wavelength.

• Notably, the upgraded cyanide-free lyse used in this process features stronger dissolving power and improved stability.